WebInstruments include the PTC Tempo, the next generation of conventional thermal cyclers, T100™, a great choice for routine PCR, and the flexible 1000-series, which offers multiple block formats for increased throughput capabilities, and a dual 48/48-well unit that allows two independent experiments to run simultaneously. Bio-Rad Thermal Cyclers Web18 Jul 2007 · PCR was performed in a DNA thermal cycler (Takara PCR thermal cycler MP, Takara Biomedical). The following primers were used: MMP-1, 5’-CGA CTC TAG AAA CAC AAG AGC AAG A-’3 (5’-primer) and 5’-AAG GTT AGC TTA CTG TCA CAC GCT T-3’ (3’-primer); MMP-2, 5’-TGT GTC TTC CCC TTC ACT TT-’3 (5’-primer) and 5’-GAT CTG AGC GAT GCC …
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WebReal-time PCR. We offer sensitive and reliable detection across a wide range of real-time PCR systems, from high-performance, to economical, to personal systems for your gene … Web30 Oct 2002 · For the PCR based on reported procedures , a mixture containing template (50 pg/μl plasmid or 3 μl of E. coli culture per 60-μl reaction), 200 μM of each dNTP, 25 units/ml ExTaq DNA polymerase (Takara), 10 nM primer-3 and primer-4, and the buffer supplied by the manufacturer was set on a Takara PCR Thermal Cycler MP (4-min denaturation at … can a crisp set be a fuzzy set
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